Mother Brain may refer to:
- The enemy from Final Fantasy II.
- The enemy from Final Fantasy Dimensions.
- The summon from Final Fantasy Dimensions II.
The name of Mother Brain refers to a central CPU with a high degree of sentience, whose appearance is often manifested as a female figure appropriate to its title. Mother Brain originated as the primary antagonist of the Nintendo series Metroid, while later series like Phantasy Star and Chrono Trigger would have their own similar but relevant take on the character archetype.